Data sheet
| Assay Range | 15.6 - 1000 pg/ml |
More info
Sensitivity
10 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human Nectin-2 standard: 5 ng/vial - 2.
2. One 96-well plate precoated with anti-human Nectin-2 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human Nectin-2 Ab: 1 vial, dilution 1:5000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Poliovirus receptor-related 2 (herpesvirus entry mediator B, nectin-2), also known as PVRL2 and CD112 (Cluster of Differentiation 112), is a transmembrane glycoprotein that functions as the receptor for alpha-herpes virus and pseudorabies virus entry into the cell. CD112 is widely expressed in neuronal, endothelial, and in particular, epithelial cells, where it is found at the intercellular junctions of adjacent cells. Additionally CD112 plays a role in the regulation of hematopoietic/endothelial cell interactions.
