Human SerpinA3 ELISA Kit (NS-E10246)
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  • Human SerpinA3 ELISA Kit (NS-E10246)

Human SerpinA3 ELISA Kit

Catalog # NS-E10246

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$625.00
Quantity

For research use only. Not for diagnostic or therapeutic use, or for human or veterinary use. Sold only to research institutions and qualified laboratory professionals. Not for sale to individuals for personal use.
Sensitivity
40 pg/mL
 
Size
96T
 
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
 
Assay Principle
Sandwich ELISA
 
Sample volume
100 µL final volume, dilution factor varies on samples.
 
Detection Method
Chromogenic
 
 
 
 
Kit Components
 
 
 
1. Recombinant Human SerpinA3 standard: 10 ng/vial - 2.
 
 
2. One 96-well plate precoated with anti-Human SerpinA3 Ab
 
 
3. Sample diluent buffer: 12 mL x 1
 
 
4. HRP conjugated anti-Human SerpinA3 Ab: 1 vial, dilution 1:400.
 
 
5. Detection Antibody diluent buffer: 12 mL x 1
 
 
6. 20 x Wash Solution: 25 ml x 1.
 
 
7. TMB color developing agent A: 6 ml.
 
 
8. TMB color developing agent B: 6 ml.
 
 
9. TMB stop solution: 6ml.

 

Background

 

Serpin A3 is also known as Alpha-1-antichymotrypsin (ACT or AACT), which belongs to the serpin family. Serpin A3 can bind DNA. SerpinA3 is an early-stage acute-phase plasma protein and a serpin that preferentially inactivates chymotrypsin, cathepsin G, and chymase. SerpinA3 can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. It appears to have a multifaceted role and is associated with inflammatory reactions, Alzheimer's disease, malignant melanoma and gastric cancer. Alpha-1-ACT, a serine protease inhibitor, is tightly associated with amyloid plaques in Alzheimer's disease and in normal aged human and monkey brain. 

Standard Curve

Data sheet

Assay Range
46.8 - 3000 pg/ml
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