Beta-Nerve Growth Factor Human Recombinant, CHO ( b NGF Human, CHO )

Data sheet

Formulation

The protein was lyophilized from a 0.2um filtered solution in 20mM PB and 250mM NaCl, pH 7.0.

Solubility

It is recommended to reconstitute the lyophilized NGF-b in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Nerve Growth Factor-beta Human Recombinant produced in CHO is a noncovalently disulfide linked homodimer, glycosylated, polypeptide chain (Ser122-Arg239) containing 2 identical 118 amino acids and having a molecular mass of 26.5 kDa.The NGF-b is purified

Protein Background

NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic ne

Expression host

Chinese Hamster Ovary Cells.

Synonyms

Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Nerve Growth Factor b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Nerve Growth Factor-beta should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term

Amino acid sequence

Was analyzed by Mass spectrometry.

Biological Activity

The ED50, calculated by its ability to stimulate chick E9 DRG neurite outgrowth was found to be 1 x 106 units/mg.