Mouse IL-17 ELISA Kit
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  • Mouse IL-17 ELISA Kit

Mouse IL-17 ELISA Kit

Catalog # NR-E10576

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$692.50
Product Size: 1x96T
Quantity

Assay Range

15.6--1000 pg/mL  

Sensitivity

1.0 pg/mL

Specificity

No cross-reaction with other related substances detected

Size

96T

Storage

Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC

Assay Principle

Sandwich ELISA

Sample Volume

100 µL final volume, dilution factor varies on samples

Sample Type

serum, plasma or cell culture supernatant 

Detection Method

Chromogenic

 

 

Kit Components

 

 1. Recombinant Mouse IL-17 standard: 1 ng x 2 vials

 2. One 96-well plate coated with Mouse IL-17  Ab

 3. Sample diluent buffer: 12 mL - 2

 4. Detection antibody: 60 µL, 1:180

 5. Streptavidin-HRP: 300 µL, 1:40 

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 6 ml TMB x1, 6 ml H2O2 x1.

 9. Stop solution: 6 mL x1

10. Washing solution (20x): 25 mL x1


For more sensitive IL-17A detection or try to save mouse plasma/serum sample volume, use more sensitive kit: Cat#NP-HS10041, http://www.novateinbio.com/home/1166285-mouse-il-17a-elisa-kit-high-sensitivity.html

 

Data sheet

Background
The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2
Alternate Names
IL17A, CTLA8, IL-17, IL-17A, IL17, CTLA-8, interleukin 17A
Gene ID
16171
UniProt ID
Q62386
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