Data sheet
| Specificity | No detectable cross-reactivity with any other cytokine. |
More info
Product name
Mouse Rantes Growth Factor ELISA Kit
Catalog No.
FM-E100120
Size
96T
Range
15.6pg/mL-1000pg/mL
Sensitivity
Less Than 1 pg/mL
Specificity
No detectable cross-reactivity with any other cytokine.
Storage
Store at 4 for frequent use, at -20 for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Expiration
Four months at 4 and eight months at -20.
Application
For quantitative detection of mouse Rantes in serum,
plasma, body fluids, tissue lysates or cell culture
supernates.
Kit Components
1. Lyophilized recombinant mouse Rantes standard: 10ng/tube—2.
2. One 96-well plate precoated with anti- mouse Rantes antibody.
3. Sample diluent buffer: 30 ml
4. Biotinylated anti- mouse Rantes antibody: 130µL, dilution 1:100.
5. Antibody diluent buffer: 12ml.
6. Avidin-Biotin-Peroxidase Complex (ABC): 130 µL, dilution 1:100.
7. ABC diluent buffer: 12ml.
8. TMB color developing agent: 10ml.
9. TMB stop solution: 10ml.
Background
Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.
Mouse Rantes ELISA Kit-1X96 Well Plate Image