Mouse P-Selectin ELISA Kit

Kit Components

 1. Recombinant  Mouse P-Selectin standard: 2 vials

 2. One 96-well plate precoated with anti-Mouse P-Selectin  Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1.

10. Washing solution (20x): 25 mL x1.

Background

P-Selectin, also known as CD62 antigen-like family member P, granule membrane protein 140 (GMP-140), leukocyte-endothelial cell adhesion molecule 3 (LECAM3), platelet activation dependent granule-external membrane protein (PADGEM), and CD62P, is a cell surface glycoprotein encoded by SELP gene in humans. P-Selectin consists of an NH2-terminal lectin type C domain, an EGF-like domain, nine complement control domains, a transmembrane domain, and a short cytoplasmic domain. The EGF-like domain of mouse P-Selectin is 92% identical to that in rat or human P-Selectin. The lectin domain of mouse P-selectin shares 92% and 76% aa sequence identity with its rat and human counterparts, respectively. P-Selectin is constitutively present in the Weibel-Palade bodies of unactivated endothelial cells and in the alpha granules of unactivated platelets. This stored P-Selectin is mobilized to the cell surface transiently in response to a variety of inflammatory stimuli.

P-selectin is a receptor for the tetrasaccharide sialyl Lewisx (sLex) which also is a ligand for both L- and E-Selectin. It is reported that P-Selectin can selectively bind to a 160 kDa glycoprotein on mouse myeloid cells and on glycoprotein present on human myeloid cells, blood neutrophils, monocytes, and lymphocytes termed P-Selectin glycoprotein ligand-1 (PSGL-1). P-selectin plays an important role in regulating the adhesion of leukocytes and neutrophils to the endothelium. A soluble form of P-Selectin (sP-Selectin), derived from alternative splicing of P-selectin mRNA, has been identified. Studies showed that elevated levels of sP-Selectin in biological fluids may be associated with certain pathological conditions.