More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 5.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse P-Selectin standard: 2 vials
2. One 96-well plate precoated with anti-Mouse P-Selectin Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
P-Selectin, also known as CD62 antigen-like family member P, granule membrane protein 140 (GMP-140), leukocyte-endothelial cell adhesion molecule 3 (LECAM3), platelet activation dependent granule-external membrane protein (PADGEM), and CD62P, is a cell surface glycoprotein encoded by SELP gene in humans. P-Selectin consists of an NH2-terminal lectin type C domain, an EGF-like domain, nine complement control domains, a transmembrane domain, and a short cytoplasmic domain. The EGF-like domain of mouse P-Selectin is 92% identical to that in rat or human P-Selectin. The lectin domain of mouse P-selectin shares 92% and 76% aa sequence identity with its rat and human counterparts, respectively. P-Selectin is constitutively present in the Weibel-Palade bodies of unactivated endothelial cells and in the alpha granules of unactivated platelets. This stored P-Selectin is mobilized to the cell surface transiently in response to a variety of inflammatory stimuli.
P-selectin is a receptor for the tetrasaccharide sialyl Lewisx (sLex) which also is a ligand for both L- and E-Selectin. It is reported that P-Selectin can selectively bind to a 160 kDa glycoprotein on mouse myeloid cells and on glycoprotein present on human myeloid cells, blood neutrophils, monocytes, and lymphocytes termed P-Selectin glycoprotein ligand-1 (PSGL-1). P-selectin plays an important role in regulating the adhesion of leukocytes and neutrophils to the endothelium. A soluble form of P-Selectin (sP-Selectin), derived from alternative splicing of P-selectin mRNA, has been identified. Studies showed that elevated levels of sP-Selectin in biological fluids may be associated with certain pathological conditions.