Data sheet
| Assay time | 4 hours |
| Validity | 6 months |
| Specificity | No detectable cross-reactivity with any other cytokine. |
| Assay Range | 31.2--2000 pg/ml |
| Sensitivity | 1.0 pg/ml |
| Size | 96T |
| Storage | 2 - 8 ℃ |
More info
Product name
Mouse IGFBP-1 ELISA Kit
Catalog No.
FM-E100048
Size
96T
Range
31.2-2,000 pg/mL
Sensitivity
3 pg/mL
Specificity
No detectable cross-reactivity with other homologs.
Storage
Store at 4 °C for frequent use, at -20°C for infrequent use.
Expiration
Six months at 4°C
Application
Sera, body fluids, tissue lysates or cell culture supernates.
Kit Components
1. Lyophilized recombinant mouse IGFBP-1 standard: 10ng/tube-2.
2. One 96-well plate precoated with anti- mouse IGFBP-1 antibody.
3. Sample diluent buffer: 30 ml
4. Biotinylated anti- mouse IGFBP-1 antibody: 130µL, dilution 1:100.
5. Antibody diluent buffer: 12ml.
6. Avidin-Biotin-Peroxidase Complex (ABC): 130µL, dilution 1:100.
7. ABC diluent buffer: 12ml.
8. TMB color developing agent: 10ml.
9. TMB stop solution: 10ml.
Background
IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb.4 The IBP-1 gene is a single copy gene, located on chromosome 7.5 The standard product used in this kit is recombinant mouse IGFBP-1, consisting of 247 amino acids with the molecular mass of 27KDa.