Globular Adiponectin Human Recombinant ( gAcrp30 Human )

Data sheet

Formulation

Sterile filtered and lyophilized from 1mg/ml in 10mM Tris pH-8 & 0.75mM DTT.

Solubility

We recommended reconstituting gAcrp30 at a concentration of 0.1-1mg per ml with 5mM Tris pH-8 and 0.75mM DTT which can then be further diluted to other aqueous solutions.

Purity

Greater than 95% as determined by SDS-PAGE.

Description

gAcrp30 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, Polypeptide chain containing 145 amino acids and having a molecular mass of 16.7kDa. The gAcrp30 is purified by proprietary chromatographic techniques.

Protein Background

gAcrp30 globular protein, exists as a result of proteolytic processing of adiponectin. Adiponectin is manufactured and secreted solely by adipocytes, and is a highly obtained plasma protein, accounting for up to 0.05% of total serum protein. Similar to Ad

Expression host

Escherichia Coli.

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Stability

For long term, store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time two weeks.

Amino acid sequence

MKGEPGEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTN.

Biological Activity

gAcrp30 activity is determined by its ability to inhibit the proliferation of mouse M1 cells. The expected ED50 for this effect is 0.36-0.54 ug/ml.