Glutathione S-Transferase Recombinant ( GST )

Data sheet

Formulation

GST supplied in Phosphate Buffered Saline pH 7.4.

Purity

Greater than 95% as determined by SDS-PAGE.

Description

Recombinant Glutathione S-Transferase full length protein (1-224a.a.) expressed in E.coli, having a molecular mass of 26kDa. GST was isolated from an E. coli strain that carries the coding sequence for Schistosoma japonicum GST under the control of a T7 p

Protein Background

Antioxidant enzyme Glutathione S- Transferase (GST) is thought to do the primary cellular defense mechanism against reactive oxygen species. GST reduces lipid hydroperoxides through its Se-independent glutathione peroxidase activity. The enzyme also detox

Expression host

Escherichia Coli.

Synonyms

Glutathione S-Transferase, GST, Glutathione S-transferase class-mu 28 kDa isozyme, GST 28, EC 2.5.1.18, Sj28GST, Sj28 antigen.

Reagent Appearance

Sterile Filtered clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Biological Activity

Specific activity is >20 units/mg. A unit is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C.