Hydroxyprostaglandin Dehydrogenase 15-( NAD ) Human Recombinant ( HPGD Human )

Data sheet

Storage

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

The HPGD protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 0.1M NaCl,1mM DTT and 20% glycerol.

Purity

Greater than 95% as determined by SDS-PAGE.

Description

HPGD Recombinant E.coli produced in E.Coli is a single, non-glycosylated polypeptide chain containing 286 amino acids (1-266a.a.) and having a molecular mass of 31.1 kDa. HPGD is fused to a 20 amino acids His-Tag at N-terminus and purified by proprietary

Protein Background

HPGD is the essential enzyme of prostaglandin degradation. 15-PGDH protein strongly decreases the biologic activity of these molecules by catalyzing the oxidation of the 15-hydroxyl group of prostaglandins to a keto group. GDH1 is involved in numerous phy

Expression host

Escherichia Coli.

Synonyms

GDH1, SDR36C1, 15-PGDH, PGDH, Prostaglandin dehydrogenase 1, hydroxyprostaglandin dehydrogenase 15-(NAD)

Reagent Appearance

Sterile filtered colorless solution.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MHVNGKVALV TGAAQGIGRA FAEALLLKGA KVALVDWNLE AGVQCKAALD EQFEPQKTLF IQCDVADQQQ LRDTFRKVVD HFGRLDILVN NAGVNNEKNW EKTLQINLVS VISGTYLGLD YMSKQNGGEG GIIINMSSLA GLMPVAQQPV YCASKHGIVG FTRSAALAAN LMNSGVRLNA ICPGFVNTAI LESIEKEENM GQYIEYKDHI KD

Antibody Properties

Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Protein content

By direct ELISA, 1:10,000 dilution will yield 0.8 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).