Beta Defensin-3 Mouse Recombinant ( BD 3 Mouse )

Data sheet

Formulation

The Mouse BD-3 was lyophilized from a 0.2um filtered concentrated solution in PBS, pH7.4.

Solubility

It is recommended to reconstitute the lyophilized Beta Defensin-3 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Beta Defensin-3 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.6kDa. The BD-3 is purified by proprietary chromatographic techniques.

Protein Background

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103A, which has broad spectrum antimicrobial activity and may play

Expression host

Escherichia Coli.

Synonyms

Beta-defensin 3, BD-3, mBD-3, Defensin beta 3, Defb3, Bd3, MGC129397.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Beta Defensin-3 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BD-3 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw c

Amino acid sequence

KKINNPVSCL RKGGRCWNRC IGNTRQIGSC GVPFLKCCKR K.

Biological Activity

Measured by its antimicrobial activity against E. coli. The ED50 for this effect is typically 4-20ug/ml, corresponding to a specific activity of 50,000-250,000units/mg.