Beta Defensin-3 Rat Recombinant ( BD 3 Rat )

Data sheet

Formulation

BD-3 protein was lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized BD-3 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

BD-3 Rat Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.5kDa.The BD-3 is purified by proprietary chromatographic techniques.

Protein Background

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103A, which has broad spectrum antimicrobial activity and may play

Expression host

Escherichia Coli.

Synonyms

Beta-defensin 3, BD-3, Defensin beta 3, Defb3.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized BD-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BD-3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a c

Amino acid sequence

KKVYNAVSCM TNGGICWLKC SGTFREIGSC GTRQLKCCKK K.

Biological Activity

Measured by its antimicrobial activity against E. coli. The ED50 for this effect is typically 4-20ug/ml, corresponding to a specific activity of 50,000-250,000units/mg.