Tumor Necrosis Factor-Alpha Human Recombinant, His Tag ( TNF a Human, His )

Data sheet

Formulation

TNF-a His is supplied in 1xPBS.

Solubility

It is recommended to reconstitute the lyophilized TNF-α in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 97.0% as determined by SDS-PAGE.

Description

Tumor Necrosis Factor-a Human Recombinant His produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids fragment (77-233) and having a molecular mass of 18.3kDa with an amino-terminal hexahistidine tag. The TNF-alpha H

Protein Background

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, diff

Expression host

Escherichia Coli.

Synonyms

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized TNF-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a

Biological Activity

EC50 0.200-0.120ng/ml measured in a metabolic inhibitor actinomycin D assay using L-cells (ATCC CCL-1).