Tumor Necrosis Factor-Alpha Porcine Recombinant ( TNF a Porcine )

Data sheet

Formulation

The protein was lyophilized with no additives.

Solubility

It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Tumor Necrosis Factor-a Porcine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17,274 Dalton. The TNF-alpha is purified by standard chromatographic techniques.

Protein Background

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, diff

Expression host

Escherichia Coli.

Synonyms

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is rec

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Ser-Ser.

Biological Activity

The ED50 as determined by the cytotoxicity of porcine PK15 cells was found to be 0.001-0.015 ng/ml.