H1N1 Influenza-A Virus Solomon Islands/03/06 Recombinant ( H1N1 Solomon Islands )

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

The H1N1 A/Solomon Islands/03/06 solution contains 0.1M NaCl, 10mM Tris-HCl, 1mM EDTA pH-8 (STE), 0.1% sodium azide (NaN3) and 0.005% thimerosal.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza A virus, strain A/Solomon Islands/03/06. The Influenza Virus was purified by Ultracentrifugation with 10-40 % sucrose gradient.

Protein Background

H1N1 is a subtype specie of Influenza A virus. H1N1 Influenza Virus has mutated into various strains such as the Spanish Flu strain, mild human flu strains, endemic pig strains, and various strains found in birds. The Influenza A Virus is a globular parti

Expression host

Baculovirus Insect Cells.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

A/Solomon Islands/03/06 although stable 4°C for 4 weeks, should be stored below -18°C. Please prevent freeze-thaw cycles.

References

Title: Heterosubtypic neutralizing antibodies are produced by individuals immunized with a seasonal influenza vaccinePublication: The Journal of clinical investigation 120.5 (2010): 1663.Link: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2860935/

Antibody Properties

Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Protein content

By direct ELISA (against recombinant gp120), 1:20,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).

Application And Suggested Dilutions

This is a binding and neutralizing antibody. Neutralization was tested in tissue culture.

Product Description

Immunoreactive with all sera of HIV-1 and HIV-type O infected individuals and with 60-80% of HIV-2 infected individuals.