Hepatitis A Virus P2C-P3B Recombinant ( HAV P2C-P3B )

Data sheet

Storage

HAV P2C-P3B Protein although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

10mM Tris-HCl, pH 9.6, 0.1% SDS and 50% glycerol.

Purity

HAV P2C-P3B protein is >90% pure as determined by 10% PAGE (coomassie staining).

Applications

HAV P2C-P3B antigen is suitable for ELISA and Western blots, excellent antigen for detection of HAV with minimal specificity problems.

Description

The E.Coli derived 53.7 kDa recombinant protein fused to a GST tag contains the P2C-P3B immunodominant regions, amino acids 1492-1606.

Protein Background

Forty-two antigenic domains were identified across the hepatitis A virus (HAV) polyprotein by using a set of 237 overlapping 20-mer synthetic peptides spanning the entire HAV polyprotein. Nineteen antigenic domains were found within the structural protein

Expression host

Escherichia Coli.

Purification Method

HAV P2C-P3B protein was purified by proprietary chromatographic technique.

Antibody Properties

Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Protein content

By direct ELISA (against recombinant gp39), 1:10,000 dilution will yield 0.3 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).