Hepatitis A Virus VP1 Recombinant ( HAV VP1 )

Catalog # PT_40931

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$315.00
DescriptionThe E.Coli derived 48 kDa recombinant protein contains the VP1 immunodominant regions, amino acids 502-605.Purification MethodHAV VP1 protein was purified by proprietary chromatographic technique.PurityHAV VP1 protein is >90% pure as
Product Size: 100ug
Quantity

Data sheet

Storage
HAV VP1 protein although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
Formulation
10mM CBB, pH9.6, 0.1% SDS and 50% glycerol.
Purity
HAV VP1 protein is >90% pure as determined by 10% PAGE (coomassie staining).
Applications
HAV VP1 antigen is suitable for ELISA and Western blots, excellent antigen for detection of HAV with minimal specificity problems.
Description
The E.Coli derived 48 kDa recombinant protein contains the VP1 immunodominant regions, amino acids 502-605.
Protein Background
Forty-two antigenic domains were identified across the hepatitis A virus (HAV) polyprotein by using a set of 237 overlapping 20-mer synthetic peptides spanning the entire HAV polyprotein. Nineteen antigenic domains were found within the structural protein
Expression host
Escherichia Coli.
Purification Method
HAV VP1 protein was purified by proprietary chromatographic technique.
Storage buffer
Km=740uM, Kcat = 30s-1, Kcat/Km=4.1mM-1s-1 with peptide substrate ATLNFPISPW.
Virus Test
20mM HEPES, pH7.0 and 10% Glycerol.
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