HIV-1 gp120 LAV Envelope Recombinant ( HIV-1 gp120 LAV )

Data sheet

Formulation

The protein solution contains 10mM Tris-Cl pH-7.6, 150mM NaCl and 0.01% Triton N-101.

Purity

Greater than 90.0% as determined by HPLC analysis and SDS-PAGE.

Applications

HIV-1 gp120 antigen is suitable for ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.

Description

HIV-1 gp120 LAV isolate Recombinant- is the external envelope protein, full-length 100-120 kDa, derived from the env. gene of HIV-1 and glycosylated with N-linked sugars and produced using baculovirus vectors in insect cells. Purified under conditions tha

Protein Background

Human immunodeficiency virus (HIV) is a retrovirusthat can lead to a condition in which the immune systembegins to fail, leading to opportunistic infections. HIV primarily infects vital cells in the humanimmune systemsuch as helper T cells(specifically CD

Expression host

Baculovirus Insect Cells.

Reagent Appearance

Sterile filtered colorless clear solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

IPGEKLWVTV YYGVPVWKEA TTTLFCASDA KAYDTEVHNV ATHACVPTDP NPQEVVLVNV TENFNMWKND MVEQMHEDII SLWDQSLKPC VKLTPLCVSL KCTDLKNDTN TNSSSGRMIM EKGEIKNCSF NISTSIRDKV QKEYAFFYKL DIVPIDNTSY RLISCNTSVI TQACPKVSFE PIPIHYCAPA GFAILKCNNK TFNGTGPCTN VSTVQCTHGI RPVVSTQLLL NG