HIV-1 nef Recombinant ( HIV-1 nef )

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

HIV-1 nef protein solution in 8M urea, 20mM Tris-HCl pH 8.0, 10mM b-mercaptoethanol.

Purity

Greater than 95.0% as determined by HPLC analysis and SDS-PAGE.

Applications

HIV-1 nef antigen in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.

Description

The E.coli derived 20kDa recombinant protein genotype IIIB is a non-glycosylated polypeptide chain, containing the HIV-1 nef immunodominant regions, 3-190 amino acids. The HIV-1 nef is fused to beta-galactosidase (114 kDa) at the N-terminus.

Protein Background

Human immunodeficiency virus (HIV) is a retrovirus that can lead to a condition in which the immune system begins to fail, leading to opportunistic infections. HIV primarily infects vital cells in the human immune system such as helper T cells (specifical

Expression host

Escherichia Coli.

Reagent Appearance

Sterile filtered colorless clear solution.

Stability

HIV-1 nef although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.

Amino acid sequence

kwskssv igwptvrerm rraepaadgv gaasqdlekh gaitssntaa tnadcawleaqeeeevgfpv tpqvplrpmt ykaavdlshf lkekgglegl ihsqrrqdil dlwiyhtqgyfpdwqnytpg pgirypltfg wcyklvpvep ekleeankge ntsllhpvsl hgmddperevlewrfdsrla.

Antibody Properties

Reconstitute with H20 to get a 1 mg/ml concentration. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Protein content

By direct ELISA, 1:20,000 dilution will yield >1.0 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).

Application And Suggested Dilutions

This antibody recognizes both human IgA1 and human IgA2 subtypes.