HIV-1 nef Clade B Recombinant ( HIV-1 nef, Clade B )

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

Lyophilized with 10% glycerol.

Solubility

It is recommended to reconstitute the lyophilized HIV-1 nef in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 90.0% as determined by HPLC analysis and SDS-PAGE.

Description

The E.coli derived 27 kDa recombinant HIV-1 Nef Clade-B protein is a single non-glycosylated polypeptide chain.

Protein Background

HIV-1 Nef is a 27kDa protein highly produced after a very short time after virus infection. HIV-1 Nef is an essential factor for efficient viral replication and phatogenesis.HIV-1 Nef facilitates virus replication and improves virions infectivity. HIV-1 N

Expression host

Escherichia Coli.

Reagent Appearance

Sterile Filtered and lyophilized, though might appear as a solution as a result of the glycerol content.

Stability

Lyophilized HIV-1 nef although stable at room temperature for 1 week, should be stored desiccated below -18°C. Upon reconstitution HIV-1 nef should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended t

Antibody Properties

Reconstitute with H20 to get a 1 mg/ml concentration. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Protein content

By direct ELISA, 1:20,000 dilution will yield >1.0 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).

Application And Suggested Dilutions

This antibody will react against all human IgA subtypes.