HIV-1 p24 gag Recombinant ( HIV-1 p24 gag )

Data sheet

Formulation

8M urea, 20mM Tris-HCl pH-8 & 10mM b-mercaptoethanol.

Purity

Greater than 95.0% as determined by HPLC analysis and SDS-PAGE.

Applications

HIV-1 p24 gag antigen in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.

Description

The E.coli derived 39 kDa recombinant protein is a non-glycosylated polypeptide chain, containing the HIV-1 p24 gag immunodominant regions, 77-436 amino acids.The HIV-1 p24 gag is fused to beta-galactosidase (114 kDa).

Protein Background

Human immunodeficiency virus (HIV) is a retrovirusthat can lead to a condition in which the immune systembegins to fail, leading to opportunistic infections. HIV primarily infects vital cells in the humanimmune systemsuch as helper T cells(specifically CD

Expression host

Escherichia Coli.

Reagent Appearance

Sterile filtered colorless clear solution.

Stability

HIV-1 p24 gag although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.

References

Title: Polyfunctional CD4+ and CD8+ T Cell Responses to Tuberculosis Antigens in HIV-1Infected Patients before and after Anti-Retroviral TreatmentPublication: The Journal of Immunology 184.11 (2010): 6537-6544.Link: http://www.jimmunol.org/content/184/1