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Data sheet
| Application | GVB++ is the traditional buffer used in complement assays. Clinical CH50 assays are performed in this buffer and almost every laboratory that performs complement assays performs them in this buffer. It is supplied as an assay-ready buffer with all the components present at the proper concentrations for CH50 assays. The concentrations of metal ions are the traditional concentrations and these give maximal complement activation. Dilutions of serum and other assay components for CH50 assays are prepared in this buffer (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)). |
| Background | Buffer for complement activity assays |
More info
For research use only. Not for use in diagnostic procedures.
Product Description
| Method: | Prepared in 18.2 megohms-cm ± 1 water and filtered through 0.22-micron filter. |
|---|---|
| Synonym: | GVB++ |
| Storage: | -20°C |
| pH: | 7.4 ± 0.15 |
| pH Tolerance: | 0.15 |
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| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Barbital | 57-44-3 | 184.19 | 5mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 145mM |
| Gelatin | 9000-70-8 | - | 0.1% |
| Calcium Chloride Dihydrate | 10035-04-8 | 147.01 | 0.15mM |
| Magnesium Chloride Hexahydrate | 7791-18-6 | 203.30 | 0.5mM |
| Water | 7732-18-5 | 18.02 | Rest |