Human uPAR ELISA Kit

Kit Components

 1. Recombinant  Human uPAR standard: 2 vials

 2. One 96-well plate precoated with anti-Human uPAR  Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1.

10. Washing solution (20x): 25 mL x1.

Background

The Urokinase receptor, also known as uPA receptor, monocyte activation antigen Mo3, uPAR or CD87, is a highly glycosylated integral membrane protein linked to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor and a receptor for urokinase-type plasminogen activator (uPA). The mature uPAR is a 283 amino acid (aa) protein derived from the full-length 335 aa uPAR precursor after removing the 22 aa signal peptide and a 30 aa fragment from the C-terminus during addition of the GPI anchor. uPAR, uPA and plasminogen activator inhibitor-1 (PAI-1) form a heterotrimetic complex that has exhibited diverse functions in regulation of cell attachment, migration, proliferation and differentiation, by both proteolytic and nonproteolytic mechanisms. There is a negative-feedback regulation by which uPAR mediates proteolysis-independent signal transduction of uPA and, in turn, uPA cleaves uPAR into an inactive form. uPAR has been involved in various non-proteolytic processes related to cancer, cell migration, cell cycle regulation, and cell adhesion.