More info
Sensitivity
15 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human TNFRSF 10B standard: 2 vials
2. One 96-well plate precoated with anti-TNFRSF 10B Ab
3. Sample diluent buffer: 12 mL x 1
4. Biotin- conjugated anti-Human TNFRSF 10B Ab: 1 vial
5. Detection Antibody diluent buffer: 12 mL x 2
6. Streptavidin-HRP: 1 vial
7. TMB color developing agent A: 6 ml
8. TMB color developing agent B: 6 ml
9. TMB stop solution: 12ml
Background
Tumor necrosis factor receptor superfamily member 10B is a member of the TNF-receptor superfamily. This receptor does not induce apoptosis, and has been shown to play an inhibitory role in TRAIL-induced cell apoptosis. Defect in TRAIL R2/CD262/TNFRSF10B is responsible for the loss of chromosome p arm which is frequently observed in head and neck tumors. TNFRSF10B inhibits tumor formation through apoptosis but deregulation encourages metastasis, migration and invasion of tumor cell tissues.
