Data sheet
| Assay Range | 15.6 - 1000 pg/ml |
More info
Sensitivity
10 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human EGFR standard: 5 ng/vial - 2.
2. One 96-well plate precoated with anti-human EGFR Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human EGFR Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
The epidermal growth factor receptor (EGFR) is is a transmembrane glycoprotein that is a member of the protein kinase superfamily. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations affecting EGFR expression or activity could result in cancer.
