Data sheet
| Assay Range | 23.44 - 1500 pg/ml |
More info
Sensitivity
20 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human ECM1 standard: 10 ng/vial - 2.
2. One 96-well plate precoated with anti-human ECM1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human ECM1 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Extracellular matrix protein 1(ECM1) is a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. ECM1 has been shown to regulate endochondral bone formation, stimulate the proliferation of endothelial cells and induce angiogenesis. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Defects in ECM1 can cause lipoid proteinosis, a disorder which causes generalised thickening of skin, mucosae and certain viscera.
