Human CXCL1/GRO alpha ELISA Kit

Kit Components

 1. Recombinant  Human CXCL1 standard: 2 vials

 2. One 96-well plate precoated with anti-Human CXCL1 Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1.

10. Washing solution (20x): 25 mL x1.

Background

CXCL1 (chemokine (C-X-C motif) ligand 1), also known as growth-regulated alpha protein, GRO-alpha, melanoma growth stimulatory activity (MGSA), or neutrophil-activating protein 3 (NAP-3), is a small cytokine belonging to the CXC chemokine family. CXCL1 is synthesized as a 107 aa precursor protein containing the N-terminal 34 aa and a 73 aa mature CXCL1. CXCL1 is expressed in monocytes, fibroblasts, melanocytes, mammary epithelial cells, and umbilical vein endothelial cells, it is induced by a variety of inflammatory mediators or growth factors, such as IL-1, TNF and PDGF. CXCL1 binds with high affinity to the type II IL-8 receptor IL-8 RB, while it binds with low affinity to the type I IL-8 receptor IL-8 RA. Recently, it has been shown that CXCL1 elicits its effects by interacting with the chemokine receptor CXCR2. CXCL1 is a potent chemoattractant for human neutrophils and stimulates neutrophil degranulation and enzyme release from cytochalasin B-treated human neutrophils. It is shown that CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis.  CXCL1 appears to induce an acute inflammatory reaction when injected into the foot-pads of endotoxin resistant mice.