Data sheet
| Assay Range | 31.25 - 2000 pg/ml |
More info
Sensitivity
30 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CHL-1 standard: 4 ng/vial - 2.
2. One 96-well plate precoated with anti-human CHL-1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CHL-1 Ab: 1 vial, dilution 1:2000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Neural cell adhesion molecule L1-like protein also known as close homolog of L1 (CHL1) is cell adhesion molecule closely related to the L1. It is an extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. It plays a role in neuronal positioning of pyramidal neurons and in regulation of both the number of interneurons and the efficacy of GABAergic synapses. This protein may play a role in regulating cell migration in nerve regeneration and cortical development.
