Data sheet
| Assay Range | 31.25 - 2000 pg/ml |
More info
Sensitivity
30 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CD97 standard: 2 ng/vial - 2.
2. One 96-well plate precoated with anti-human CD97 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CD97 Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
CD97, also known as Leukocyte antigen CD97and CD97 antigen, is a protein that belongs to the adhesion-GPCR receptor family. CD97 is normally found on tissue of hematapoetic origin and in smooth muscle. N-terminal domains of CD97 consist of alternatively spliced epidermal growth factor (EGF)-like domains which mediate binding to its cellular ligand, decay accelerating factor (DAF/CD55), a regulatory protein of the complement cascade. CD97 has been found to be upregulated in Cancers, including prostate, breast, and thyroid. In prostate cancer, it has been shown that CD97 is hetoerodimerizing with another GPCR, LPAR. The interaction is believed to further enhance the RHO signaling between both receptors.
