Data sheet
| Assay Range | 78.125 - 5000 pg/ml |
More info
Sensitivity
50 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CD8A standard: 10 ng/vial - 2.
2. One 96-well plate precoated with anti-human CD8A Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CD8A Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
CD8a (Cluster of Differentiation 8a), is a cell surface glycoprotein. The CD8 antigen is found on most cytotoxic T lymphocytes that mediate efficient cell-cell interactions within the immune system, acting as a coreceptor. CD8A Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 alpha chains bind to class I MHC molecules alpha-3 domains. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
