More info
Assay Range | 15.6-1,000 pg/mL |
Sensitivity | 1.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human TGFalpha standard: 2 vials
2. One 96-well plate coated with anti- Human TGFalpha Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1
Background
Transforming growth factor alpha (TGF-α), also known as EGF-like TGF (ETGF), TGF type 1, is a member of the EGF family. TGF-α gene encodes a 160 amino acid (aa) precursor protein consisting of an N-terminal, plasma membrane-targeting signal sequence, extracellular domain with N- and O-linked glycosylation sites, a single transmembrane-spanning region, and a cytoplasmic tail. Human TGF-α shares approximately 93% aa sequence identity with mouse and rat counterparts, respectively.
TGF-α is widely expressed in various tissues such as endocrine, hematopoietic, immune, integumentary, nervous, respiratory, and urinary systems. TGF-α precursor is cleaved by tumor necrosis factor alpha converting enzyme (TACE) to generate the soluble mature TGF-α which is a ligand for the EGFR. TGF-α binding initiates EGF R dimerization, tyrosine autophosphorylation, and activation of signaling pathways involving SH domain containing proteins to regulate transcription. Primarily, TGF-α plays a role in promoting cell proliferation, differentiation, and transformation. Recently, it has been showed that TGF-α also functions in angiogenesis, bone resorption, cell metabolism, cell migration and wound healing.