Data sheet
| Specificity | No detectable cross-reactivity with any other cytokine. |
More info
Product name
Human IL-6 ELISA Kit
Catalog No.
FM-E100066
Size
96T
Range
4.69pg/mL-300pg/mL
Sensitivity
Less Than 0.3 pg/mL
Specificity
No detectable cross-reactivity with any other cytokine.
Storage
Store at 4 °C for frequent use, at -20 °C for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Expiration
Four months at 4 °C and eight months at -20 °C .
Application
serum, plasma, body fluids, tissue lysates or cell culture supernates.
Kit Components
1. Lyophilized recombinant human IL-6 standard: 300 pg/tube×2.
2. One 96-well plate precoated with anti- human IL-6 antibody.
3. Sample diluent buffer: 12 ml x 2
4. Biotinylated anti- human IL-6 antibody : 180μl, dilution 1:60.
5. Antibody diluent buffer: 12 ml.
6. Streptavidin-HRP: 300μl, dilution 1:40.
7. Streptavidin-HRP diluent buffer: 12 ml.
8. TMB color developing agent: 12 ml.
9. TMB stop solution: 6 ml.
Background
The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids.6 The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.
Human IL-6 ELISA Kit-1X96 Well Plate Image