Human IGF-2 ELISA Kit

Human IGF-2 ELISA Kit

FM-E100044

96T

62.5pg/mL-4000pg/mL

Less Than 2pg/mL

No detectable cross-reactivity with IGF-1.

Store at 4 for frequent use, at -20 for infrequent use.

Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Four months at 4 and eight months at -20.

serum, plasma, body fluids, tissue lysates or cell culture supernates.

 

 

1. Lyophilized recombinant human IGF-2 standard: 10ng/tube-2.

2. One 96-well plate precoated with anti- human IGF-2 antibody.

3. Sample diluent buffer: 30 ml

4. Biotinylated anti- human IGF-2 antibody: 130µL, dilution 1:100.

5. Antibody diluent buffer: 12ml.

6. Avidin-Biotin-Peroxidase Complex (ABC): 130µL, dilution 1:100.

7. ABC diluent buffer: 12ml.

8. TMB color developing agent: 10ml.

9. TMB stop solution: 10ml.

 

 

Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland. The human IGF-II gene is located on chromosome 11. The standard product used in this kit is recombinant human IGF-2, consisting of 67 amino acids in mature form with the molecular mass of 7.5KDa. IGF-1 and IGF-2 share high homology.

 

Human IGF-2 ELISA Kit-1X96 Well Plate Image