Human E-Selectin ELISA Kit

Human E-Selectin ELISA Kit

FM-E100122

96T

62.5pg/mL-4000pg/mL

Less Than 4 pg/mL

No detectable cross-reactivity with any other cytokine.

Store at 4 for frequent use, at -20 for infrequent use.

Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Four months at 4 and eight months at -20.

For quantitative detection of human E-Selectin in

cell culture supernates, serum and plasma(heparin, citrate).

1. Lyophilized recombinant human E-Selectin standard: 10ng/tube×2.

2. One 96-well plate precoated with anti- human E-Selectin antibody.

3. Sample diluent buffer: 30 ml

4. Biotinylated anti- human E-Selectin antibody: 130μl, dilution 1:100.

5. Antibody diluent buffer: 12ml.

6. Avidin-Biotin-Peroxidase Complex (ABC): 130μl, dilution 1:100.

7. ABC diluent buffer: 12ml.

8. TMB color developing agent: 10ml.

9. TMB stop solution: 10ml.

E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant human E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 535 amino acids with the molecular mass of 58.6KDa.

Human E-Selectin ELISA Kit-1X96 Well Plate Image