Data sheet
| Specificity | No detectable cross-reactivity with any other cytokine. |
| Sensitivity | < 4 pg/ml |
| Size | 96T |
| Storage | Store at 4 for frequent use, at -20 for infrequent use. |
| Expiration | Four months at 4 and eight months at -20. |
| Application | For quantitative detection of human E-Selectin in |
| Range | 62.5pg/ml-4000pg/ml |
More info
Product name
Human E-Selectin ELISA Kit
Catalog No.
FM-E100122
Size
96T
Range
62.5pg/mL-4000pg/mL
Sensitivity
Less Than 4 pg/mL
Specificity
No detectable cross-reactivity with any other cytokine.
Storage
Store at 4 for frequent use, at -20 for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Expiration
Four months at 4 and eight months at -20.
Application
For quantitative detection of human E-Selectin in
cell culture supernates, serum and plasma(heparin, citrate).
Kit Components
1. Lyophilized recombinant human E-Selectin standard: 10ng/tube×2.
2. One 96-well plate precoated with anti- human E-Selectin antibody.
3. Sample diluent buffer: 30 ml
4. Biotinylated anti- human E-Selectin antibody: 130μl, dilution 1:100.
5. Antibody diluent buffer: 12ml.
6. Avidin-Biotin-Peroxidase Complex (ABC): 130μl, dilution 1:100.
7. ABC diluent buffer: 12ml.
8. TMB color developing agent: 10ml.
9. TMB stop solution: 10ml.
Background
E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant human E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 535 amino acids with the molecular mass of 58.6KDa.
Human E-Selectin ELISA Kit-1X96 Well Plate Image