Human CD40L ELISA Kit

Human CD40L ELISA Kit

FM-E100157

96T

62.5pg/mL-4000pg/mL

Less Than 15 pg/mL

No detectable cross-reactivity with any other cytokine.

Store at 4 for frequent use, at -20 for infrequent use.

Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Four months at 4 and eight months at -20.

For quantitative detection of human CD40L in

cell culture supernates, serum and plasma(heparin, EDTA)

 

1. Lyophilized recombinant human CD40L standard: 10ng/tube-2.

2. One 96-well plate precoated with anti- human CD40L antibody.

3. Sample diluent buffer: 30 ml

4. Biotinylated anti- human CD40L antibody: 130µL, dilution 1:100.

5. Antibody diluent buffer: 12ml.

6. Avidin-Biotin-Peroxidase Complex (ABC): 130µL, dilution 1:100.

7. ABC diluent buffer: 12ml.

8. TMB color developing agent: 10ml.

9. TMB stop solution: 10ml.

 

 

CD40 ligand(CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb /IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.

 

Human CD40L ELISA Kit-1X96 Well Plate Image