Data sheet
| Assay Range | 6.25 - 400 pg/ml |
More info
Sensitivity
5 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Mouse CD209b standard:1 ng/vial - 2.
2. One 96-well plate precoated with anti-Mouse CD209b Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Mouse CD209b Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
CD209 antigen-like protein B, also known as DC-SIGN-related protein 1, DC-SIGNR1 and Cd209b, is a type II transmembrane C-type lectin. It is expressed at high levels in splenic marginal zone macrophages and lymph node medullary macrophages. CD209b is also expressed in skin and lung. It may mediate the endocytosis of pathogens, which are subsequently degraded in lysosomal compartments and binds to mannose-like carbohydrates as a receptor for ICAM3. In addition, it has been recently shown that SIGNR1 deficient mice have a defect in catabolism of the complement component C3, and that SIGNR1 binds directly to the complement C1 subcomponent, C1q to assemble a non-conventional C3 converses.
