Kallikrein-1 Human Recombinant ( KLK1 Human )

Data sheet

Formulation

Lyophilized from a solution containing 1xPBS.

Solubility

It is recommended to reconstitute the lyophilized KLK1 in sterile 18M-cm H2O not less than 100 ug/ml or more than 10 mg/ml solutions.

Purity

Greater than 98.0% as determined by both:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

KLK1 Human Recombinant produced in Yeast is a single, glycosylated, polypeptide chain containing 238 amino acids and having a molecular mass of 28-32 kDa.KLK1 Human Recombinant is purified by proprietary chromatographic techniques.

Protein Background

Kallikreins are serine protease enzymes having various physiological functions. Kallikreins are implicated in carcinogenesis and have potenital as novel cancer disease biomarkers. KLK1 is one of the fifteen kallikrein subfamily members located in a cluste

Expression host

Pichia Pastoris.

Synonyms

KLK1, KLK-1, HK1, HK-1, KLKR, KLK6, Tissue Kallikrein, hKLK1, EC 3.4.21.35, Kidney/pancreas/salivary gland kallikrein, Kallikrein-1.

Reagent Appearance

Sterile Filtered white lyophilized powder.

Stability

Lyophilized KLK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution KLK1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a ca

Biological Activity

Human Kallikrein-1 active Unit Definitin: 1 unit will hydrolyze 1 umole of H-D-Val-Leu-Arg-pNA(S-2266) per minute at pH 8.0 at 37°C Human Kallikrein-1 measured biological activity of no less than 5 Units/mg (S2266 method).

Protein concentration

Can be used for lateral follow product, ELISA assay and vaccine development.