Thrombopoietin Human Recombinant, HEK ( TPO Human, HEK )

Data sheet

Formulation

The TPO protein was filtered (0.2um) and lyophilized from 0.73mg/ml in 1xPBS.

Solubility

It is recommended to reconstitute the lyophilized Thrombopoietin in sterile PBS containing 0.1% endotoxin-free recombinant HSA.

Purity

Greater than 95% as obsereved by SDS-PAGE.

Description

Thrombopoietin Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 80-85kDa due to glycosylation.The TPO is purified by proprietary chromatographic techniques.

Protein Background

Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that fragment int

Expression host

HEK.

Synonyms

Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, C-mpl ligand, ML, Megakaryocyte growth and development factor, MGDF, TPO, MKCSF, MPLLG, MGC163194, THPO.

Stability

Lyophilized Thrombopoietin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TPO should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to

Physical Appearence

Sterile Filtered White lyophilized (freeze-dried) powder.

Biological Activity

The activity was determined by the dose-dependent stimulation of the proliferation of MO7e cells, the EC50 is 3.8ng/ml.