Fibroblast Growth Factor-Basic Human Recombinant, Plant ( FGF 2 Human, Plant )

Data sheet

Formulation

FGF-b was lyophilized from a concentrated solution without any additives.

Solubility

It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

FGF-2 Human Recombinant produced in rice is a single, non-glycosylated polypeptide chain containing 146 amino acids and having a molecular mass of ~17kDa.The FGF-b protein is purified by proprietary chromatographic techniques.

Protein Background

Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell

Expression host

Rice Grain (Oryza Sativa).

Synonyms

Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b, Fibroblast growth factor 2, Basic fibroblast growth factor, Heparin-binding growth factor 2.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-b should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is

Biological Activity

The ED50, as calculated by the dose-dependent proliferation of Balb/c 3T3 cells expressing FGF receptors is 1 ng/ml, corresponding to a specific activity of >1 x106 Units/mg.