Fibroblast Growth Factor-Acidic Human Recombinant, Sf9 ( FGF 1 Human, Sf9 )

Data sheet

Formulation

The sterile protein solution (1.8mg/ml) contains 20mM Tris HCl pH=7.9, 100mM KCl, 0.2mM DTT and 20% glycerol.

Purity

Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Fibroblast Growth Factor-1 Human Recombinant (FGF-1) produced in Sf9 insect cells is a single, glycosylated, polypeptide chain containing 140 amino acids and having a molecular mass of 15803 Dalton. The FGF-a is purified by proprietary chromatographic tec

Protein Background

Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell

Expression host

Baculovirus.

Synonyms

HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a.

Reagent Appearance

Sterile Filtered liquid formulation.

Stability

Fibroblast Growth Factor-acidic although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Amino acid sequence

The sequence of the first five N-terminal amino acids as determined and was found to be Met-Phe-Asn-Leu-Pro.

Biological Activity

The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is