Fibroblast Growth Factor-4 Human Recombinant, HEK ( FGF4 Human, HEK )

Data sheet

Formulation

The FGF-4 was lyophilized from 1mg/ml in 1xPBS.

Solubility

It is recommended to reconstitute the lyophilized FGF4 in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95% as obsereved by SDS-PAGE.

Inactivation

Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.15 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program o

Description

FGF-4 Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 17-27kDa due to glycosylation.The FGF4 is purified by proprietary chromatographic techniques.

Protein Background

FGF4 holds s comprehensive mitogenic and cell survival activities and takes part in a range of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF4 possess oncogenic transforming

Expression host

HEK.

Synonyms

HBGF4, FGF-4, FGF4, KFGF, HSTF1.

Stability

Lyophilized FGF-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF4 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a c

Physical Appearence

Sterile Filtered White lyophilized (freeze-dried) powder.

Biological Activity

The specific activity was determined by the dose-dependent stimulation of the proliferation of the Balb/3T3 cell line and is typically 0.25-1.25ng/ml.