Fibroblast Growth Factor-basic Human Recombinant, HEK ( FGF 2 Human, HEK )

Data sheet

Formulation

The FGF2 was filtered (0.2um) and lyophilized from 0.68mg/ml in 1xPBS.

Solubility

It is recommended to reconstitute the lyophilized FGF-b in sterile PBS containing 0.1% endotoxin-free recombinant HSA.

Purity

Greater than 95% as obsereved by SDS-PAGE.

Description

FGF-2 Human Recombinant Thermostable produced in HEK cells is a non-glycosylated monomer, containing 154 amino acids and having a total molecular weight of 17kDa.FGF-2 Thermostable is a protein engineered FGF2 in order to enhance its thermostability witho

Protein Background

Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell

Expression host

HEK.

Synonyms

Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.

Stability

Lyophilized FGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-b should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a

Physical Appearence

Sterile Filtered White lyophilized (freeze-dried) powder.

Biological Activity

The specific activity was determined by the dose dependent stimulation of proliferation of the Balb/c 3T3 cell line, the ED50 is 0.007ng/ml.