Growth Hormone Porcine Recombinant ( GH Porcine )

Data sheet

Formulation

The Growth-hormone (1 mg/ml) was lyophilized after extensive dialyses against 0.34 mg sodium phosphate buffer (0.02 mg sodium phosphate monobasic & 0.32 mg sodium phosphate dibasic).

Solubility

It is recommended to reconstitute the lyophilized pST in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Porcine-Somatotropin Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 190 amino acids and having a molecular mass of 21730 Dalton. Growth Hormone is purified by proprietary chromatographic techniques.

Protein Background

GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same

Expression host

Escherichia Coli.

Synonyms

GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin, pST.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized GH although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Somatotropin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to ad

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Phe-Pro-Ala-Met-Pro.

Biological Activity

Somatotropin contains 3 units/mg.

Contaminants

Western Blotting: 1g/ml.