Hepatocyte Growth Factor Human Recombinant, HEK ( HGF Human, HEK )

Data sheet

Formulation

The HGF was lyophilized from 1mg/ml in 1xPBS.

Solubility

It is recommended to reconstitute the lyophilized HGF in sterile PBS containing 0.1% endotoxin-free recombinant HSA.

Purity

Greater than 95% as obsereved by SDS-PAGE.

Description

HGF Human Recombinant produced in HEK cells is a glycosylated peptide, containing 697 a.a. (Gln-32 to Ser-728) having a total molecular weight of 70kDa.The HGF is purified by proprietary chromatographic techniques.

Protein Background

Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulat

Expression host

HEK.

Synonyms

Scatter Factor (SF), Hepatopoietin (HPTA), HGF, HGFB, F-TCF.

Stability

Lyophilized HGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carr

Amino acid sequence

32-QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR

Physical Appearence

Sterile Filtered White lyophilized (freeze-dried) powder.

Biological Activity

The specific activity was determined by the dose-dependent stimulation of the proliferation of the monkey epithelial cell line 4MBr-5 and is typically 10-50ng/ml.