Insulin-Like Growth Factor 1 Rat Recombinant ( IGF1 Rat )

Data sheet

Formulation

The protein was lyophilized with a 0.2um filtered concentrated solution in 20mM PBS, pH 7.0.

Solubility

It is recommended to reconstitute the lyophilized IGF1 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 98.0% as determined by SDS-PAGE.

Description

Insulin-Like Growth Factor I Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.7kDa. IGF-I is purified by proprietary chromatographic techniques.

Protein Background

The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early studies show

Expression host

Escherichia Coli.

Synonyms

Somatomedin C, IGF-I, IGFIA, IGF1.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized IGF-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFI should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a c

Amino acid sequence

GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKSA.

Biological Activity

The ED50 as determined by a cell proliferation assay using FDC-P1 cells is less than 2.0ng/ml, corresponding to a specific activity of >500,000units/mg.

References

Title:Evidence that androgen-independent stromal growth factor signals promote androgen-insensitive prostate cancer cell growth in vivo.Publication: Published online before print March 17, 2009, doi: 10.1677/ERC-08-0219 Endocr Relat Cancer June 1, 2009 16