Insulin-Like Growth Factor-2 Human Recombinant ( IGF2 Human )

Data sheet

Formulation

The protein was lyophilized with no additives.

Solubility

It is recommended to reconstitute the lyophilized IGF2 in sterile 18M-cm H2O at a concentration of 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 97.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Insulin-Like Growth Factor-II Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 67 amino acids and having a molecular mass of 7505 Dalton. IGF-II is purified by proprietary chromatographic techniques.

Protein Background

IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a r

Expression host

Escherichia Coli.

Synonyms

Somatomedin-A, IGF2, INSIGF, pp9974, C11orf43, FLJ22066, FLJ44734.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized IGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFII should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a

Amino acid sequence

AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE.

Biological Activity

The ED50, calculated by the dose-dependent induction of MCF-7 cell proliferation was found to be 0.85ng/ml corresponding to a Specific Activity of 1,176,470IU/mg.

References

Title: Sub-Cellular Localisation Studies May Spuriously Detect the Yes-Associated Protein, YAP Publications: Nucleoli Leading to Potentially Invalid Conclusions of Its Function. PLoS ONE 10.2 (2015): e0114813. Link: http://journals.plos.org/plosone/artic