Insulin-Like Growth Factor Binding Protein-5 Human Recombinant ( IGFBP 5 Human )

Catalog # PT_41116

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$201.00
DescriptionIGFBP5 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 253 amino acids and having a molecular mass of 28613 Dalton. IGFBP5 is purified by proprietary chromatographic
Product Size: 5ug
Quantity

Data sheet

Reconstitution
1mg/ml in PBS (after reconstitution).
Formulation
IBP-5 was lyophilized from a concentrated (1mg/ml) solution containing 10mM sodium Citrate pH-3.
Solubility
It is recommended to reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-5 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Purity
Greater than 96.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Description
IGFBP5 Human Recombinant produced in E.Coli is non-glycosylated homodimer containing 2x252 amino acids and having a molecular mass of 28.6kDa. IGFBP5 is purified by proprietary chromatographic techniques.
Protein Background
IGFBP5 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFA
Expression host
Escherichia Coli.
Synonyms
IGFBP-5, IBP-5, IGF-binding protein 5.
Reagent Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Stability
Lyophilized IBP5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFBP 5 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a
Amino acid sequence
LGSFVHCEPC DEKALSMCPP SPLGCELVKE PGCGCCMTCA LAEGQSCGVY TERCAQGLRC LPRQDEEKPL HALLHGRGVC LNEKSYREQV KIERDSREHE EPTTSEMAEE TYSPKIFRPK HTRISELKAE AVKKDRRKKL TQSKFVGGAE NTAHPRIISA PEMRQESEQG PCRRHMEASL QELKASPRMV PRAVYLPNCD RKGFYKRKQC KPSRGRKRGI CWCVDKYGMK LP
Biological Activity
The ED50, calculated by its ability to inhibit IGF-II induced proliferation of MCF-7. The expected ED50 for this effect is 2500 IU/mg in the presence of 15ng/ml of rHuIGF-II.
References
Title:Effects of Dietary Carbohydrate Modificationin Persons with the Metabolic Syndrome- A Transcriptomics Approach in Adipose TissuePublication:Department of Clinical Nutrition, Food and Health Research CentreInstitute of Public Health and Clinical Nutr
Antibody Properties
Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Titer
Antibody is shipped lyophilized at ambient temperature.
Introductionn
This antibody will stain 70% of PHA-activated human T cells (by FACS). 10ul will stain 106 IL-2R positive cells for FACS analysis or sorting.
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