Insulin-Like Growth Factor Binding Protein-6 Human Recombinant ( IGFBP 6 Human )

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

IBP-6 was lyophilized from a concentrated (1mg/ml) solution containing 1xPBS, 0.1% SDS and 1mM DTT.

Solubility

It is recommended to reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-6 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 80.0% as determined by SDS-PAGE.

Description

IGFBP6 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing amino acids 148-240 and having a molecular mass of 20 kDa including 4kDa His tag. IGFBP6 is purified by proprietary chromatographic techniques.

Protein Background

IGFBP6 plays a role in lipoprotein assembly and dietary cholesterol absorption. in addition to its acyltransferase activity, it may act as a ligase. may provide cholesteryl esters for lipoprotein secretion from hepatocytes and intestinal mucosa.

Expression host

Escherichia Coli.

Synonyms

IGFBP-6, IBP-6, IGF-binding protein 6.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized IBP6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGFBP-6 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a

Antibody Properties

Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Introductionn

This antibody will stain 70% of PHA-activated human T cells (by FACS). 10ul will stain 106 IL-2R positive cells for FACS analysis or sorting.