Keratinocyte Growth Factor Human Recombinant, HEK ( KGF Human, HEK )

Data sheet

Formulation

The KGF was lyophilized from 1mg/ml in 1xPBS.

Solubility

It is recommended to reconstitute the lyophilized KGF in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95% as obsereved by SDS-PAGE.

Description

KGF Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 17-30kDa due to glycosylation.The KGF is purified by proprietary chromatographic techniques.

Protein Background

KGF is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tiss

Expression host

HEK.

Synonyms

HBGF-7, FGF7, FGF-7, KGF.

Stability

Lyophilized KGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution KGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carr

Physical Appearence

Sterile Filtered White lyophilized (freeze-dried) powder.

Biological Activity

The specific activity was determined by the dose-dependent stimulation of the proliferation of 4MBr-5 cells (monkey epithelial cell line) and is typically 1.5-7.5ng/ml corresponding to a Specific Activity of 133,334-666,667IU/mg.