Leptin Human Recombinant, His Tag ( Leptin Human, His )

Data sheet

Formulation

The protein was lyophilized from a concentrated (1mg/ml) solution with 1X PBS, 0.1% SDS and 1mM DTT.

Solubility

The lyophilized Leptin is very soluble in water and most aqueous buffers below and above the isoelectric point.

Purity

Greater than 90.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Applications

1. Positive control for Western blot.2. Antibody production. 3. Protein assay.

Description

Leptin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing amino acids 48-167 and having a total molecular mass of 19 kDa including the 4 kDa His tag.The Leptin is purified by proprietary chromatographic techni

Protein Background

Leptin is a protein hormone with important effects in regulating body weight, metabolism and reproductive function. The protein is approximately~16 kDa in mass and encoded by the obese (ob)gene. leptin is expressed predominantly by adipocytes, which fits

Expression host

Escherichia Coli.

Synonyms

OB Protein, Obesity Protein, OBS, Obesity factor.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Leptin although stable at room temperature, should be stored desiccated below 0°C. Reconstituted Leptin is best stored refrigerated at 4°C.Please avoid freeze-thaw cycles.